Characterization of 2,3,7,8-tetrachlorodibenzo-p-dioxin biodegradation by extracellular lignin-modifying enzymes from ligninolytic fungus
نویسندگان
چکیده
Ligninolytic fungi secrete extracellular lignin-modifying enzymes (LME) that degrade plant polymers for fungal nutrition but are, because of their broad substrate specificity, also applicable the degradation many hazardous pollutants. Laccase is one most well characterized LME and involved in removal recalcitrant aromatic compounds with or without assistance laccase-mediators. The fungus Rigidoporus sp. FMD21 can 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) a half-life 6.2 days. Using crude enzyme extract (ExE) mainly consisted laccase, 77.4% 2,3,7,8-TCDD was degraded within 36 rate did not depend on concentration tested range between 0.005 0.5 pgTEQ/?L. analysed by DR-CALUX® bioassay confirmed GC-HRMS. In this study, we found evidence cleavage diaryl ether bond molecule here propose new mechanism which 3,4-dichlorophenol main metabolite FMD21’s ExE. Six laccase-mediators were tested. Three them 1-hydroxybenzotriazole (HBT), syringaldehyde (Syr) violuric acid (Vio) showed an equipotent added effect ExE, however only case Vio level significance reached. others no negatively impacted degradation. conclusion, have shown produces enzymes, laccases apparently are able to highly toxic 2,3,7,8-congener TCDD via into 3,4-dichlorophenol.
منابع مشابه
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ژورنال
عنوان ژورنال: Chemosphere
سال: 2021
ISSN: ['0045-6535', '1879-1298']
DOI: https://doi.org/10.1016/j.chemosphere.2020.128280